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EasyClone cDNA Library Construction Kit

Yeast Media Protein Expression Purification
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EasyClone cDNA Library Construction Kit (Cat # P01010)

 

 

Quickly construct full-length, highly representative cDNA libraries for the DUALmembrane and DUALhybrid systems! EasyClone cDNA synthesis is based on a novel technology utilizing the specific features of MMLV-based reverse transcriptase to create full length enriched double stranded cDNA from total or polyA+ RNA samples. cDNAs are amplified by representational PCR and cloned directionally into DUALmembrane or DUALhybrid library vectors for use with Dualsystems' protein interaction screening kits. No extensive know-how in library construction is needed - the kit supplies all reagents needed for library construction and the detailed manual will guide you through all steps of construction.

  • Fast, easy synthesis of highly complex, representational cDNAs
  • High representation of full length transcripts
  • Directional cloning into DUALmembrane and DUALhybrid vectors
  • Only small starting amounts of RNA required
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