DUALmembrane System

DUALmembrane Starter Kits

• Protein-Protein Interaction Screening
  
  
• Protein Expression
  
  
• Protein Extraction and Refolding
  
  
• Library Construction and Preparation
  
  
• Yeast Kits, Strains and Tools
  
  
• Anti-Yeast & DNA Repair Antibodies
  
  
• Custom Services

Click here for a listing of all combinations of bait kit plus library!

The DUALmembrane (Stagljar et al., 1998; Thaminy et al., 2003) system takes advantage of the split-ubiquitin mechanism (Johnsson and Varshavsky,1994) to measure the interaction between an integral membrane protein and its interaction partners (either integral membrane proteins or a soluble proteins).

A first membrane protein of interest (the bait) is fused to the C-terminal half of ubiquitin (Cub) and the artificial transcription factor LexA-VP16. A second protein of interest (the prey) is fused to the mutated N-terminal half of ubiquitin (NubG). If bait and prey interact NubG and Cub are forced into close proximity, resulting in the reconstitution of split-ubiquitin. Split ubiquitin is immediately recognized by ubiquitin specific proteases that then cleave the polypeptide chain between Cub and LexA-VP16. As a result, the artificial transcription factor is released from the membrane and translocates to the nucleus where it binds to the LexA operators situated upstream of a reporter gene via its LexA DNA binding domain.

The DUALmembrane system features:

• The highly sensitive yeast host strain NMY51 features three reporter genes for superior screening stringency
• Personalize your kit by selecting your bait vector and library!
• Two control prey constructs and one control bait construct are included
• Transformation reagents and trial-size beta-gal assay kit included

DUALmembrane Pairwise Interaction Kit (Cat# P01501)

DUALmembrane Vector Complementation Set (Cat# P01502)
(available only when ordered with the DUALmembrane starter kit SUC)

Western Blotting Control Extracts (Cat# P06002)
Control extracts prepared from the yeast strain DSY-5 transformed with either a control bait plasmid or a control prey plasmid. Perfect for checking the sensitivity of your Western blot and troubleshooting the quality of total yeast extracts. Each set contains 50 ml (enough for 5 loads) of each: Control bait extract (DSY-5 transformed with pMBV1-Ost1), and Control prey extract (DSY-5 transformed with pAI-Alg5)

View Premade Libraries for the DUALmembrane/DUALhunter System