DUALrefold System

DUALrefold Soluble Protein Refolding Kit (Cat # P07201)

DUALrefold Membrane Protein Refolding Kit (Cat # P07501)

• Protein-Protein Interaction Screening
  
  
• Protein Expression
  
  
• Protein Extraction and Refolding
  
  
• Library Construction and Preparation
  
  
• Yeast Kits, Strains and Tools
  
  
• Anti-Yeast & DNA Repair Antibodies
  
  
• Custom Services

DUALrefold Soluble Protein Refolding Kit (Cat # P07201)
Using the DUALrefold Soluble Protein Refolding Kit, optimal protein folding conditions can be identified by a simple bench-top centrifugation and incubation procedure. No tedious dialysis or high ratio dilution is needed. No special equipment or protein folding experience is required to use the folding kit. About 0.06 - 0.12 mg of inactive protein can be used per refolding assay. Once optimal re-folding conditions are identified using each of the 10 columns in the kit, individual DUALrefold columns can be purchased for either small scale (0.6 - 1.2 mg) or large scale (5 - 10 mg) purification and refolding preparations.

DUALrefold Membrane Protein Refolding Kit (Cat # P07501)
The DUALrefold membrane protein refolding kit is specifically designed for optimizing the refolding of membrane proteins from urea-solubilized inclusion bodies or other protein aggregates. Using the supplied spin columns and reagents, 20 different refolding conditions (including various detergents and lipids that form micelles and bicelles) can be tested. The micellar and bicellar environments facilitate folding receptors, ion channels and other membrane proteins. Once optimal re-folding conditions are identified using each of the 20 columns in the kit, individual DUALrefold columns can be purchased for large scale (5 - 10 mg) purification of urea solubilized membrane proteins.