TOTAL Protein Extraction Kit

TOTAL Protein Extraction Kit (Cat # P01013)

• Protein-Protein Interaction Screening
  
  
• Protein Expression
  
  
• Protein Extraction and Refolding
  
  
• Library Construction and Preparation
  
  
• Yeast Kits, Strains and Tools
  
  
• Anti-Yeast & DNA Repair Antibodies
  
  
• Custom Services

The TOTAL Protein Extraction Kit has been specially formulated for efficient extraction and solubilization of total protein mixtures from fungi and bacteria. After mechanical disruption of the cell wall by glass beads, proteins are solubilized using a complex detergent mix. The use of several detergents ensures uniform solubilization of proteins with widely different characteristics, including strongly hydrophobic proteins such as integral membrane proteins. After solubilization, proteins are denatured by addition of sample buffer and loaded onto an SDS-PAGE gel for further analysis.

The kit is recommended for preparation of total protein extracts in DUALhybrid, DUALmembrane and DUALhunter screens and is also compatible with other yeast two-hybrid systems.