QuickLyse Expression System

QuickLyse pEAS-1a Expression Vector (Cat # P08001)

• Protein-Protein Interaction Screening
  
  
• Protein Expression
  
  
• Protein Extraction and Refolding
  
  
• Library Construction and Preparation
  
  
• Yeast Kits, Strains and Tools
  
  
• Anti-Yeast & DNA Repair Antibodies
  
  
• Custom Services

The novel QuickLyse vector pEAS-1a provides rapid, temperature-controlled autolysis of bacteria expressing your recombinant protein of interest.

Simply clone your gene into pEAS-1a, transform into an appropriate expression host and induce protein expression. Autolysis is induced via an increase in temperature. After lysis, recombinant protein is immediately accessible for purification directly from the culture supernatant.

With the QuickLyse vector, there is no need for lysozyme treatment or sonication, and gentle extraction conditions ensure integrity and activity of the recombinant protein. The QuickLyse system uses the autolytic genes derived from the SRRz cluster of phage Lambda (Kloos et al., 1994). The SRRz genes are under the control of the heat inducible cI857/pR promoter from phage Lambda and the strong rrnB terminator. Shifting cells from 28°C to 38°C induces expression of the SRRz genes, resulting in quick and efficient autolysis of bacterial cells.

The system is compatible with common E. coli expression strains such as BL21 or XL1-Blue.