Antibodies

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Mouse Monoclonal anti-LexA Antibody (Cat # P06004)
The Mouse monoclonal anti-LexA Antibody recognizes the DNA binding domain of the E. coli SOS repressor protein LexA. This antibody has been specifically tested for Western blotting applications involving the detection of LexA fusion proteins from total yeast extracts. It is suitable for detecting both yeast two-hybrid and DUALmembrane fusion proteins.

Recommended dilution for Western blotting is 1:5,000. Dilute antibody in 1x PBS pH 7.4, 0.1% Tween-20, 1% non-fat milk powder, or similar incubation buffer. We recommend incubation volumes of 10 ml and incubation times of 10-15 hours at 4°C.

Provided at 40 ul, 200 ug/ml IgG. Sufficient for 20 Western blots. Store at 4C, do not freeze.


Mouse Monoclonal anti-HA Antibody (Cat # P06005)
The mouse monoclonal antibody (IgG1), directed against the HA epitope tag, has been specifically tested for Western blotting applications involving the detection of HA fusion proteins from total yeast extracts. It is suitable for detecting both yeast two-hybrid and DUALmembrane fusion proteins.

Recommended dilution for Western blotting is 1:5,000. Dilute antibody in 1x PBS pH 7.4, 0.1% Tween-20, 1% non-fat milk powder, or similar incubation buffer. We recommend incubation volumes of 10 ml and incubation times of 10-15 hours at 4°C.

Provided at 20 ul, 5 ug/ml IgG. Sufficient for 10 Western blots. Store at 4C, do not freeze.

Mouse Monoclonal anti-c-myc Antibody (Cat # P06006)
This mouse monoclonal antibody (IgG) has been specifically tested for Western blotting applications involving the detection of c-myc fusion proteins in total yeast extracts. It is also suitable for detecting recombinant proteins expressed in Escherichia coli and in a variety of mammalian cell lines and primary cells.

Recommended dilution for Western blotting is 1:7’500. Dilute antibody in 1x PBS pH 7.4, 0.1% Tween-20, 1% non-fat milk powder, or similar incubation buffer. We recommend incubation volumes of 10 ml and incubation times of 10-15 hours at 4°C.

Provided at 30 ul, 400 ug/ml IgG. Sufficient for 20 Western blots. Store at 4C, do not freeze.