Yeast Host Strains

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NMY51 Yeast Strain (Cat # P04005)
Optimized screening strain for conventional yeast two-hybrid and DUALmembrane screens. Two growth markers and a lacZ reporter ensure the highest possible stringency in a library screen, resulting in fewer false positives. This strain is part of the DUALhybrid and DUALmembrane 3 kits.
MATa his3delta200 trp1-901 leu2-3,112 ade2 LYS2::(lexAop)4-HIS3 ura3::(lexAop)8-lacZ (lexAop)8-ADE2 GAL4)

NMY61 Yeast Strain (Cat # P04006)
Mating partner for reporter strain NMY51.
MATalpha his3delta200 trp1-901 leu2-3,112 ade2 LYS2::(lexAop)4-HIS3 ura3::(lexAop)8-lacZ (lexAop)8-ADE2 GAL4)

DSY-5 Yeast Strain (Cat # P04003)
General purpose strain for expression of recombinant proteins in yeast. Deletion of the two major vacuolar proteases Pep4 and Prb1 ensures good yield and little non-specific degradation when purifying proteins from yeast. Can be used in combination with our general purpose yeast expression vectors.
MATa leu2 trp1 ura3-52 his3 pep4 prb1

DSY-6 Yeast Strain (Cat # P04007)
DSY-6 has been designed for expression of recombinant proteins in yeast. Good growth characteristics ensure high cell densities both in synthetic defined (SD) and rich (YPD) medium. Deletion of prb1 encoding the vacuolar proteinase B (yscB), pep4 encoding the vacuolar aspartyl protease (proteinase A) and prc1 encoding the vacuolar carboxypeptidase Y (proteinase C) ensures minimal protein degradation during cell lysis and protein extraction.
MATa leu2 trp1delta63 ura3-52 prb1-22 pep4-3 prc1-407