Yeast Nitrogen Bases

YNB +/- Nitrogen

• Acronyms, Terminology and FAQs
  
  
• Amino Acid Dropout Mixtures
  
  
• Media for S. cerevisiae
  
  
• Media for S. pombe
  
  
• Supplements, Additives and Raw Materials
  
  
• Custom Media

Yeast Nitrogen Base (YNB) contains salts, vitamins and trace elements as defined below. Add 1.7 g/L of YNB to ammonium sulfate (5g/L), dextrose (20g/L) and an amino acid mix to make a complete media.

Potassium phosphate (1000 mg/L), Inositol (2 mg/L), Copper sulfate (0.04 mg/L), Magnesium sulfate (500 mg/L), Niacin (0.4 mg/L), Potassium iodide (0.1 mg/L), Sodium chloride (100 mg/L), PABA (0.2 mg/L), Ferric chloride (0.2 mg/L), Calcium chloride (100 mg/L), Pyridoxine (0.4 mg/L), Manganese sulfate (0.4 mg/L), Biotin (0.002 mg/L), Riboflavin (0.2 mg/L), Sodium molybdate (0.2 mg/L), Pantothenate, calcium (0.4 mg/L), Thiamine (0.4 mg/L), Zinc sulfate (0.4 mg/L), Folic acid (0.002 mg/L), Boric acid (0.5 mg/L)

Burkhalter media contains YNB (1.7 g/L), ammonium sulfate (5 g/L), citric acid (6.1 g/L), and sodium phosphate (20.2 g/L).